Validating internal controls for quantitative plant gene expression studies Sexi chat with bianca

Posted by / 11-Sep-2017 06:43

Validating internal controls for quantitative plant gene expression studies

Furthermore, it is easy to use, allows high throughput production of data and eliminates the need for radioactive isotopes [].

Thus, the understanding of some key genes' expression patterns will help illuminate the mechanism involved in this process.Thus, a suitable HKG should be selected before its use.Only a few studies on HKGs have been done in plants, and none in soybean, an economically important crop.The HKGs, which are referred to as internal controls or reference genes, are presumed to have constant expression level among different tissues and at all developmental stages, regardless of the experimental conditions or treatments.Additionally, the HKG and target gene should have similar transcript levels to avoid analytical problems [], such as components of the cytoskeleton (actins), glycolytic pathway (glyceraldehyde-3-phosphate dehydrogenase (GAPDH)), protein folding (cyclophilin), synthesis of ribosome subunits (r RNA), electron transporter (succinate dehydrogenase complex, SDH), protein degradation (ubiquitin), etc.

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Therefore, the present study was conducted to identify suitable HKG(s) for normalization of gene expression in soybean.

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